Application of an ELISA for detection of antibodies in sera of goats sensitized by killed brucella melitensis

Sera were collected at weekly intervals from goats sensitized by killed Brucella melitensis strain 16M and from a control non sensitized goats. ELISA using 2 types of antigens and 2 types of conjugates were applied on goat sera along with 3 conventional serological tests. Comparison between ELISA antigens and conjugates were studied. ELISA using KCl extract of Brucella melitensis as an antigen and anticaprine IgG as a conjugate proved to be superior that other tests in detection of antibodies against Brucella melitensis specially in late period after sensitization